Regulatory

Part:BBa_K090502:Design

Designed by: Daniel Goodman   Group: iGEM08_Cambridge   (2008-10-27)


Gram-Positive Xylose-Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the following PCR primers to extract the part, sans biobrick ends: Forward: TTCATGAAAAACTAAAAAAAATATTG Reverse: TATGTCATATTGTAAGTAAGTTGCAC


Source

We PCRed this part out of the pSG1154 gram-positive shuttle vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE153.

References